The authors investigated total serum LD activity and isoenzymes in 5 cases of acute
hepatitis, 14 cases of chronic hepatitis, 25 cases of liver cirrhosis, 17 cases of
hepatocellular carcinoma and 20 cases of healthy control. The sera were obtained from
the anterior cubital veins of the patients and healthy control in Busan National
University Hospital from June 1978 to July 1979. Total Serum LD activity was
measured by method of Caboud-Wroblewski and isoenzyme fractions were analysed by
cellulose acetate electrophoresis.
The results obtained were as follows :
1) In the patients with liver diseases, total serum LD activity was significantly
increases than that of healthy controls.
2) In the patients with liver diseases LD1 and LD2 were
significantly decreased than those of healtly controls, while LD4 and
LD5 were significantly increased.
3) The patterns of serum LD isoenzyme fractions showed LD2>
LD1,>LD3>LD4>LD5, in healtly
adult, LD5>LD2>LD1>LD3>
LD4in acute hepatitis and LD2>LD1>
LD3>LD5>LD4 in chronic hepatitis, liver
cirrhosis and hepatocellular carcinoma.
4) In the patients with liver diseases, the ratio of
LD1+LD2/LD4+LD5X100 was
significantly decreased than that of healthy control.
5) The diagram for the percent distribution of serum LD isoenzymes demonstrated that
all the fractions of LD isoenzymes except LD3: in acute hepatitis and
LD5 in other liver diseases were out range (M±2 SD) of healthy control.
On the basis of above, the authors consider that total serum LD activity, isoenzymes
and diagram for the percent distribution of isoenzymes may be help of clinically in
making diagnosis and differention of various liver diseases.
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